Fig. 6: The positive feedback loop between PPARγ and P38 promotes NET production. | Nature Communications

Fig. 6: The positive feedback loop between PPARγ and P38 promotes NET production.

From: Residual tumor cells after insufficient radiofrequency ablation promote lung metastasis by educating CD177hiPAD4hi neutrophils

Fig. 6: The positive feedback loop between PPARγ and P38 promotes NET production.

A mTOR, p-mTOR, Akt, p-Akt, P38, and p-P38 proteins detected in CT26 cells at 37 °C/45 °C ± OA. B Representative GSEA analysis of the MAPK pathway in scRNA-seq data from untreated and iRFA-treated CT26 tumors (n = 3 mice). Kolmogorov-Smirnov test. FDR correction. C P38 and p-P38 in untreated and iRFA-treated tumor tissues. The quantification shown on the right (n = 9 biological replicates). ANOVA. D Nuclear PPARγ, and cytosol CD36, FABP4, and FABP5 proteins in CT26 cells treated with DMSO or SB202190 at 37 °C and 45 °C + OA. E FA levels in CT26/DLD1 cells treated with DMSO, SB190, or SB580 at 37 °C and 45 °C + OA (n = 3 biological replicates). P < 0.001, ANOVA. F Nile red staining of CT26 cells treated with DMSO or SB190 at 37 °C and 45 °C + OA. G p-P38 and P38 in CT26 cells treated with siCtrl or siPparg at 37 °C and 45 °C + OA (Upper), and treated with DMSO or T0070907 (Lower). H p-P38 and P38 proteins in CT26 and DLD1 cells treated with DMSO or PF at 37 °C and 45 °C + OA. I CD36, FABP4, FABP5, PPARγ, and p-P38 staining in liver tumors from iRFA and Ctrl mice. J Pearson correlation between P38 and neutrophil infiltration. K P38 score and neutrophil infiltration score of each patient obtained through ssGSEA. Survival analysis in NEUHp38H and NEULwp38L patients. L MPO-dsDNA levels in neutrophil co-cultured with conditioned media from CT26/DLD1 cells treated with DMSO or SB190 at 37 °C, 37 °C + OA, 45 °C, and 45 °C + OA (n = 3 biological replicates), P < 0.001, ANOVA. M Lung weight of iRFA and Ctrl CT26 mice treated with SB190 or PBS (n = 5 mice), P < 0.001, ANOVA. N HE staining of lung tumors. Scale bars, 1 mm and 50 μm. O Immunofluorescence detection of Ly6G and cit-H3 in liver tumors from iRFA-CT26 mice treated with SB190 or PBS. Scale bars, 50 μm. Data are presented as mean values ± SD. A, D, G, and H 37/45: 37 °C/45 °C. E, F, L, M, and O SB190: SB202190. E SB580: SB203580. H PF: PF-04620110. Source data are provided as a Source Data file.

Back to article page