Fig. 2: SQGro is metabolized by R. denitrificans OCh 114 and A. macleodii ATCC 27126. | Nature Communications

Fig. 2: SQGro is metabolized by R. denitrificans OCh 114 and A. macleodii ATCC 27126.

From: Cosmopolitan marine bacteria facilitate a vast phytoplankton-derived sulfonate-based carbon flow through sulfoquinovosidases

Fig. 2

a Growth curve of R. denitrificans OCh 114 on SQGro (A600 represents optical density, concentration refers to SQGro). A600 (C1) (orange) indicates a control group with only bacteria added and no SQGro. A600 (C2) (black) represents a control group containing SQGro and no bacteria, with concentration of SQGro shown by SQGro (C) (blue). A600 (T) (red) represents the experimental group with both SQGro and bacteria, with concentration of SQGro shown by SQGro (T) (green). Data represent means ± SD (n = 3 biological replicates). b Growth curve of A. macleodii ATCC 27126 on SQGro. Optical density of the medium (orange), SQGro concentration in the medium (purple), sulfate concentration in the medium (green), and sulfite concentration in the medium (blue). Data represent means ± SD (n = 3 biological replicates). c Scatter plot of comparative proteomic analysis of A. macleodii ATCC 27126 cultured with SQGro + yeast extract vs. yeast extract. Red dots represent proteins that are increased in abundance (p < 0.05, t-test, two-sided) in SQGro + yeast extract growth conditions, whereas blue dots represent those proteins that are significantly decreased in abundance (p < 0.05, t-test, two-sided). The proteins marked by gene locus are increased in abundance at least tenfold. The protein ID (WP_014951134.1) corresponds to the gene locus tag (MASE_18025). The statistical details are provided in Supplementary Data 2. d Enzymatic activity assay measured for AmSqgH catalyzed hydrolysis of SQGro. LC-MS extracted ion chromatograph of SQ with m/z 243.0170 after the reaction. e MS-MS fragmentation of SQ product showing HSO3−, C4H7O6S−, and C3H5O5S− ions. f Enzyme activity measured for AmSqgH catalyzed hydrolysis of PNPSQ. Data represent means ± SD (n = 3 biological replicates). g Activity plot for PNPSQ hydrolysis by AmSqgH from A. macleodii ATCC 27126 showing fit to Michaelis–Menten equation. Data represent means ± SD (n = 3 biological replicates). h Predicted 3D structure of AmSqgH (green) superimposed on that of EcYihQ (pink, PDB code: 5AEE) and AtSQase (purple, PDB code: 5OHS) and expansion showing β-sheet connection within EcYihQ and AtSQase. Source data are provided as a Source Data file.

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