Fig. 3: Phylogenetic analysis of GH31 subfamily distribution. | Nature Communications

Fig. 3: Phylogenetic analysis of GH31 subfamily distribution.

From: Cosmopolitan marine bacteria facilitate a vast phytoplankton-derived sulfonate-based carbon flow through sulfoquinovosidases

Fig. 3

Phylogenetic tree (constructed using IQ-TREE) of selected species containing examples of all predicted GH31 subfamily protein sequences (shown at right). The predicted sulfoquinovosidase function of SqgH clade and GH31_13 subfamily protein sequences were annotated according to the proposed SQ metabolic pathway for the gene cluster in which they are located. Numbers at nodes correspond to bootstrap support for major nodes over 60%. Insert: AmSqgH, as well as proteins from GH31_3 (named GH31_3, from E. coli K-12, gene locus: Ga0198492_01_4502455_4504773) and GH31_6 (named GH31_6, from Achromobacter aegrifaciens, gene locus: RA224_28300) subfamily were used to test catalytic activity on PNPSQ. AmSqgH were used to test catalytic activity on PNPXyl and PNPGlc, and SDS-PAGE analyses of the purified GH31_3 and GH31_6. Data represent means ± SD (n = 3 biological replicates). Pathway abbreviations: sulfo-EMP sulfoglycolytic Embden-Meyerhof-Parnas, sulfo-EMP2 a variant of the sulfo-EMP pathway, sulfo-ED sulfoglycolytic Entner-Doudoroff, sulfo-TK sulfoglycolytic sulfoquinovose transketolase, sulfo-TAL sulfoglycolytic transaldolase pathways96. Source data are provided as a Source Data file.

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