Fig. 2: Cryo-EM structures of the D-loop complexes with various homologous dsDNA.
From: RAD51 D-loop structures reveal the mechanism of eukaryotic RAD51-mediated strand exchange
a–e Cryo-EM reconstructions of RAD51 D-loop complexes assembled with donor dsDNA substrates containing 4-bp, 8-bp, another 8-bp, 8-bp, and 12-bp mismatched bubbles, respectively. For each panel, a schematic of the ssDNA and the corresponding dsDNA mismatched bubble is shown on the left, and the corresponding cryo-EM density map is shown on the right. RAD51 protomers are labeled from N + 1 to N + 9 along the filament axis. In the density maps, the N-terminal domain (NTD), displaced strand, and complementary strand are colored orange, green, and blue, respectively. The global resolution of each reconstruction is indicated in the respective panel.
