Fig. 2: Targeted DNA methylation at the FWA promoter is heritable.
From: Versatile molecular tools enabling customizable DNA methylation editing in Arabidopsis
a Dot plot of rosette leaf count at flowering-time of indicated T2 plants in the fwa background. Red dots indicate the corresponding T2 plants lacking the transgene (the same below); black dots indicate the corresponding T2 plants containing the transgene (the same below); gray dots represent untransformed plants in the indicated background (the same below). The dashed line corresponds to the leaf number threshold (21) below which plants are considered early-flowering relative to fwa plants. The code names referring to the tools are listed below. The controls and T2 plants were grown side by side. b Bar graph showing FWA expression level in the indicated T2 and control plants. “+” indicates T2 plants containing the transgene (the same below); “-” indicates T2 plants without the transgene (the same below). The controls and T2 plants were grown side by side. Error bars are defined as the standard deviation (SD) of three technical replicates; data are presented as mean ± s.d.. c Screenshot of DNA methylation over FWA promoter in the indicated T2 plants. Please note that the control WGBS data for fwa-1 and fwa-2 used here are identical to those used in Fig. 1f. Source data are provided as a Source data file.
