Fig. 3: Targeted DNA methylation at the FT enhancer Block C.
From: Versatile molecular tools enabling customizable DNA methylation editing in Arabidopsis
a Schematic of targeting DNA methylation at the FT enhancer Block C. DNA methylation at FT enhancer Block C induces a late-flowering phenotype. b Dot plot of rosette leaf count at flowering-time of T1 plants transformed with the indicated tools, compared to control plants. The dashed line corresponds to the leaf number threshold (15) above which plants are considered delayed flowering relative to WT plants. The controls and T1 plants were grown side by side. c WT plants and representative late-flowering T1 plants expressing MQ1v-dCas9 (M14) to illustrate differences in flowering time. d Screenshot of DNA methylation at the FT enhancer Block C in the indicated control, T1, and T2 plants. ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) shows the position of enhancer Block C. The controls and T1 plants were grown side by side. e Dot plot of rosette leaf count at flowering-time of the indicated T2 plants. The dashed line corresponds to the leaf number threshold (15) above which plants are considered delayed flowering relative to WT plants. The controls and T2 plants were grown side by side. Source data are provided as a Source data file.
