Fig. 4: Targeted demethylation at the FWA promoter.
From: Versatile molecular tools enabling customizable DNA methylation editing in Arabidopsis
a Schematic of targeting demethylation at the FWA promoter. b WT plants and representative late-flowering T1 plants expressing TET1cd-dCas9 (D12) to illustrate differences in flowering time. c Bar graph showing FWA expression level relative to ACT2 in representative late-flowering T1 plants expressing the indicated tools and control plants. The controls and T1 plants were grown side by side. Error bars are defined as the SD of three technical replicates; data are presented as mean ± s.d.. d Dot plot of rosette leaf count at flowering-time of T1 plants transformed with the indicated tools, compared to control plants. The dashed line corresponds to the leaf number threshold (15) above which plants are considered delayed flowering relative to WT plants. The controls and T1 plants were grown side by side. e Screenshot of DNA methylation over FWA promoter in the indicated T1 plants. The controls and T1 plants were grown side by side. f Genome-wide CG methylation levels in the indicated T1 and WT plants. Source data are provided as a Source data file.
