Fig. 2: Transgenic mini-yellow restores mosquito pigmentation. | Nature Communications

Fig. 2: Transgenic mini-yellow restores mosquito pigmentation.

From: Mosquito sex separation using complementation of selectable traits and engineered neo-sex chromosomes

Fig. 2

A Structure of EnYR (Endogenous yellow rescue) and HspYR (Heatshock yellow rescue) rescue constructs containing the mini-yellow coding sequence (CDS) driven either by endogenous yellow regulatory elements or the hsp83 promoter and P10 terminator. Both constructs also contain the OpIE2:eGFP transformation marker and piggyBac arms. B Crossing scheme to test complementation. Transgenic males homozygous for the native yellow (y+) allele were crossed to homozygous yellow (y-) mutant females, producing F1 progeny heterozygous at the yellow (y+/y-) locus and carrying the mini-yellow rescue construct. F1 transgenic males were then test-crossed with homozygous yellow (y-) mutant females to evaluate pigmentation restoration in F2 progeny. As a control non-transgenic F1 heterozygous males were crossed to homozygous y- females. C Distribution of pigmentation phenotypes for dark and yellow among transgenic and non-transgenic individuals. Significant reduction from a default 50% yellow (z range: –4.82 to –8.38; p < 0.00000146, two-tailed z-test for proportions) is indicated. Source data are provided as a Source Data file.

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