Fig. 1: Septic shock is induced in a dose-dependent manner by injection of pathogenic E. coli J96 and is linked to platelet microvascular deposition and a drop in circulating platelet count. | Nature Communications

Fig. 1: Septic shock is induced in a dose-dependent manner by injection of pathogenic E. coli J96 and is linked to platelet microvascular deposition and a drop in circulating platelet count.

From: Platelet-mediated activation of perivascular mast cells triggers progression of sepsis to septic shock in mice

Fig. 1

A Mortality rates after iv injection of 1×107, 5×107, 1×108, and 4×108 colony forming units/ml (CFU) E. coli J96 (n = 6 mice/group; p = 0.0053). B Correspondence of invasively measured blood pressure (in black) and rectal temperature (in red). Arrow indicates timepoint of iv injection of 4×108 CFU E. coli J96 (n = 5 mice/group). C Platelet count after iv injection of saline (Sham; n = 7), or of E. coli J96 at 1 × 108 (Sepsis; n = 4), or 4×108 CFU (Shock; n = 11). D Representative tissue immunofluorescence staining of whole mount ear skin for vasculature (CD31, blue), platelets (CD41, green), and the monocytes/neutrophil marker GR1 (red) in sham-treated mice and after injection of 4×108 CFU E. coli J96. CD41 (yellow triangle) and GR1 positive (white triangle) cell clusters were averaged from 5 fields per slide (n=9mice/group). Significance determined by Log-rank test, one- or two-way ANOVA (with Tukey’s multiple comparisons test), or one-sided, unpaired Student’s t test as appropriate. All data are shown as mean ± SEM. Boxplot outlines the median (central line), the interquartile range (boxes), and 1.5 times the interquartile range (whiskers). Source data are provided as a Source Data file.

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