Fig. 6: MC activation is critical for development of microcirculatory abnormalities in septic shock. | Nature Communications

Fig. 6: MC activation is critical for development of microcirculatory abnormalities in septic shock.

From: Platelet-mediated activation of perivascular mast cells triggers progression of sepsis to septic shock in mice

Fig. 6

A Representative photoacoustic (PA) images from mouse ear skin vasculature at baseline and 30 minutes after injection of 4×108 colony forming units/ml (CFU) E. coli J96 into WT mice (n = 5). Close ups (Scale bar: 200 μm) detail areas outlined by yellow box in upper images (Scale bar: 1 mm). Triangles highlight loss of small vessel perfusion in septic shock. B Relative vessel area measured as average PA signal amplitude over the entire mouse ear before and during the first 40 minutes after injection of 4×108 CFU E. coli J96 into WT (WT+Shock), MC-deficient mice (KitW-sh+Shock), and WT mice pretreated with the MC inhibitor Ketotifen (Ketotifen+Shock). C Quantification of relative vessel area for Baseline (BL) and 30 minutes after E. coli J96 injection (Shock). D Hemoglobin oxygen saturation (sO2) responses from same area as in A before and 30 min after injection of E. coli J96. E sO2 responses over time after injection of E. coli J96 for conditions as outlined in B). F Quantification of relative oxygenation for Baseline (BL) and 30 min after E. coli J96 injection (Shock). Significance determined one-way ANOVA (with Tukey’s multiple comparisons test). All data are shown as mean ± SEM. Source data are provided as a Source Data file.

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