Fig. 1: The high levels of glycolysis and lactate are correlated to the delayed graft function (DGF). | Nature Communications

Fig. 1: The high levels of glycolysis and lactate are correlated to the delayed graft function (DGF).

From: Nucleophosmin 1 lactylation in graft kidney induces ferroptotic trigger waves that exacerbate delayed graft function

Fig. 1: The high levels of glycolysis and lactate are correlated to the delayed graft function (DGF).

a Heatmap illustrated the energy metabolic metabolites in mouse kidney tissues after ischemia and reperfusion for 0 h, 12 h and 24 h (n = 4 group−1). b Lactate assay kit detecting lactate concentration in IRI kidney tissues at different reperfusion stages (n = 6 group−1) and normalized according to the sham group concentrations; one-way ANOVA followed by Tukey’s test. c, d The translational and transcriptional levels of glycolytic enzymes in mouse kidney tissues following ischemia-reperfusion injury (IRI) (n = 6 group−1), and normalized according to sham group levels; one-way ANOVA followed by Tukey’s test. e, f The serum creatinine (SCr) and blood urea nitrogen (BUN) concentration of mice after kidney IRI (n = 6 group−1), and one-way ANOVA followed by Tukey’s test; Boxplot: 25th–75th percentiles; center line: median; whiskers: 1.5 times the interquartile range. g A two-sided Pearson correlation analysis of SCr concentration and lactate concentration (r = 0.756, p < 0.001, n = 30). h, i Urinary lactate concentration of non-DGF (n = 105) and DGF (n = 45) recipients on the postoperative days 2 (ULa-2 d) and 7 (ULa-7 d); unpaired 2-tailed Student’s t test. j A two-sided Pearson correlation analysis of SCr and ULa-2 d concentrations (r = 0.716, p < 0.001, n = 150). k The ROC curve of the DGF prediction model based on ULa-2 d of recipients in the training set (n = 150). l, m Urinary lactate concentration of non-DGF (n = 45) and DGF (n = 19) recipients in the validation set and the ROC curve of the DGF prediction model; unpaired 2-tailed Student’s t test. n Kaplan‒Meier survival curves of the five-year graft survival stratified by the median ULa-2 d level (n = 110); the p value was calculated via the log-rank test. Data are presented as mean ± SD. All experiments were repeated at least three times, yielding similar results. The pvalues are shown for the indicated comparisons. Source data are provided as a Source Data file.

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