Fig. 7: Pdk1 upregulation, protein translation and JAK/STAT signalling are linked in tumour growth.
From: A JAK/STAT-Pdk1-S6K axis bypasses systemic growth restrictions to promote regeneration
A, B Control (A) and wing disc expressing RasV12, scrib-RNAi (B) for 44 h starting at Day6 AED. OPP incorporation (cyan/grey) and JAK/STAT-reporter 10xStat92E-dGFP (magenta/grey). C Mean OPP intensity in the 10xStat92E-dGFP positive and negative region within a RasV12, scrib-RNAi-expressing wing discs. Mean and 95% CI, two-tailed Paired t test, p-value = 0.0006 (control: n = 6, experiment: n = 6). D, E Control and wing disc expressing RasV12, scrib-RNAi. Pdk1-GFP (cyan/grey) in control (D) and RasV12, scrib-RNAi-expressing wing disc (E), along with MMP1 staining to visualises JNK-activity (magenta/grey). F Mean Pdk1-GFP intensity in the control pouch and RasV12, scrib-RNAi-expressing pouch. Mean and 95% CI, two-tailed Unaired t test, p-value = 0.0004 (control: n = 10, experiment: n = 10). G, H OPP incorporation in the notum of control (G) and wing disc expressing RasV12, scrib-RNAi (H). I Mean OPP intensity in the notum of control and wing disc expressing RasV12, scrib-RNAi. Mean and 95% CI, two-tailed Welch’s t test, p-value = 0.0031 (control: n = 6, experiment: n = 6). Scale bars: 100 μm. DAPI visualises nuclei. Fluorescence intensities are reported as arbitrary units. Source data in graphs are provided as a Source Data file.
