Fig. 3: In vivo characterization of NSP15H234A.
From: SARS-CoV-2 EndoU-ribonuclease regulates RNA recombination and impacts viral fitness
a Schematic of golden Syrian hamster infection with WT or H234A SARS-CoV-2. Three-to-four-week-old golden Syrian hamsters were intranasally infected with 105 plaque forming units (PFU) of WT or H234A and monitored for weight changes and signs of disease over a 7-day time course. Hamsters were nasal washed, and lung lobes were collected at days 2, 4, and 7 post infection for viral titers and histopathology analyses. Created in BioRender. Menacherylab, T. (2025) https://BioRender.com/6ula8ve. b Percent body weight change from starting weight for WT or H234A infected groups. Viral titers were measured for nasal wash (c) and lung (d) for animals infected with WT or H234A at day 2, 4, and 7 post infection. b–d Data are presented as mean ± SD. Statistical analysis was measured using a two-tailed Student’s t-test. Statistics are conducted with two-tailed Student’s t-test, α = 0.05, N = 5 biological replicates. ND: not detected.
