Fig. 5: NSP15H234A resulted in elevated host immune responses during early infection.
From: SARS-CoV-2 EndoU-ribonuclease regulates RNA recombination and impacts viral fitness
a Hierarchical gene clusters based on average read count of each gene. H234A and WT showed distinctive gene expression profiles with particular clusters of genes (red box) at day 2 (D2) but not at day 4 (D4). b Principal component assay (PCA) showed that host responses against H234A progressed faster than WT at D2 but comparable at D4. c Up- and down-regulated genes between H234A and WT at D2 (Padj < 0.1, |log2FC| > 0.585, N = 3 biological replicates). d Top 10 gene ontology (GO) terms discovered from significantly (Padj < 0.1, |log2FC| > 1, N = 3 biological replicates) up- or down- regulated genes (WT vs H234A) at D2. e At D2, WT and H234A showed both correlated and anti-corelated gene expression profiles in comparison to mock. Red box: genes that are up-regulated for both WT and H234A; blue box: genes that are down-regulated by WT but up-regulated by H234A. f RNA fold expression (Log2 transformed) of individual innate immune genes in infected WT (black) and H234A (red) relative to time-matched mock hamster lungs. Data are presented as mean ± SD. Statistics are conducted with two-tailed Student’s t-test, α = 0.05, N = 3 biological replicates.
