Fig. 4: Analysis of disease-related PGT mutations.
From: Molecular basis of prostaglandin E2 reuptake by organic anion transporter PGT
a Structural mapping of genetic variants identified in PHO and CEAS patients. b Expanded view of the region covering G222R and P219L. c Relative activity (%WT) of G222R and P219L at 80 nM of PGE2. Activities are normalized to that of WT PGT (mean ± SEM, n = 3 biologically independent experiments, with three technical replicates in each experiment), based on their relative surface expression level assessed using in-gel fluorescence (Supplementary Fig. 8). d PGE2 binding affinity measured for the two mutants (mean ± SEM, n = 3 independent experiments). Source data are provided as a Source Data file.
