Fig. 3: Cryo-EM structure of nb.C11 bound to NEDD4-2 HECT domain (WW4-HECT4-2). | Nature Communications

Fig. 3: Cryo-EM structure of nb.C11 bound to NEDD4-2 HECT domain (WW4-HECT4-2).

From: Ion channel inhibition by targeted recruitment of NEDD4-2 with divalent nanobodies

Fig. 3: Cryo-EM structure of nb.C11 bound to NEDD4-2 HECT domain (WW4-HECT4-2).The alternative text for this image may have been generated using AI.

a Final cryo-EM map of the WW4-HECT4-2:nb.C11 complex at 3.1 Å resolution (EMD-73821). Sharpened density map is contoured at 0.124. b Atomic model of WW4-HECT4-2:nb.C11 complex (PDB 9Z5Q) including a close-up view showing NEDD4-2 N-lobe small subdomain (NLSSD) interactions with CDR1-3 loops on nb.C11. c Superposition of previously published E2 enzyme (UBCH5B) (PDB: 3JW0; red), donor ubiquitin (UbD) (PDB: 3JW0; dark brown) and acceptor ubiquitin (UbA) (PDB: 4BBN; light brown) binding sites on the HECT4-2:nb.C11 complex (HECT4-2, light gray; nb.C11, dark gray).

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