Fig. 8: Comparative impact of NEDD4-2 over-expression and DiVa recruitment of endogenous NEDD4-2 on the proteome. | Nature Communications

Fig. 8: Comparative impact of NEDD4-2 over-expression and DiVa recruitment of endogenous NEDD4-2 on the proteome.

From: Ion channel inhibition by targeted recruitment of NEDD4-2 with divalent nanobodies

Fig. 8: Comparative impact of NEDD4-2 over-expression and DiVa recruitment of endogenous NEDD4-2 on the proteome.The alternative text for this image may have been generated using AI.

a Schematic of experimental flow chart for tandem mass tag (TMT) mass spectrometry analyses of HEK293 cells proteome-wide changes. Partially created in BioRender. Colecraft, H. (https://BioRender.com/jd6uc7h) (b–d) Volcano plots comparing proteomes of HEK293 cells expressing (b) KCNQ1-YFP + nb.YFP vs KCNQ1-YFP + NEDD4-2, c KCNQ1-YFP + nb.YFP vs KCNQ1-YFP + DiVa nb[YFP-C11], and (d) KCNQ1-YFP + DiVa nb[YFP-C11] vs KCNQ1-YFP + NEDD4-2. Dotted lines demarcate fold-change (FC) cut-off = 1.5 and p-value = 0.05 using Welch’s two-sided unpaired t-test. Down-regulated genes fitting the cut-off criteria are colored in red and up-regulated genes in green. e, f Top terms from GO analyses of down-regulated and up-regulated genes, respectively. Adjusted p-values obtained from Welch’s two-sided unpaired t-test followed by BH-FDR adjustment.

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