Fig. 7: Caveolae assembly precedes RhoA activation at contraction sites. | Nature Communications

Fig. 7: Caveolae assembly precedes RhoA activation at contraction sites.

From: Spatiotemporal coupling of caveolae mechanosensing and RhoA-GEFs regulates cell polarity and directional migration

Fig. 7: Caveolae assembly precedes RhoA activation at contraction sites.

a Representative time-lapse images of WT Hs578t cells expressing wild-type Cav1-RFP and a RhoA activity biosensor (RhoA-bio), showing a random contraction event. b Quantification of normalized intensity over time for RhoA-bio (purple), Cav1 (orange), and cell area (blue) at contraction sites. Data are presented as mean values ± SEM., n = 109 contraction events from 45 cells pooled from 3 independent experiments. c Time-lapse images of cavin1-KO cells under the same conditions. d Corresponding quantification of normalized RhoA-bio, Cav1, and cell area. Data are presented as mean values ± SEM., n = 68 contraction events from 45 cells from 3 independent experiments. e Time-lapse images of Cav1-KO cells re-expressing wtCav1 and RhoA-bio. f Data are presented as mean values ± SEM., n = 73 contraction events from 45 cells from 3 independent experiments. Scale bars, 10, µm. Fluo., fluorescence. Source numerical data are provided.

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