Table 1 Cryo-EM data collection, model refinement and validation statistics
From: Molecular insights into ligand recognition and signaling of OXGR1
Data collection and processing | Oxoglutarate-GPR99/OXGR1-Gq | LTE4-GPR99/OXGR1-Gq |
|---|---|---|
Magnification | 105,000 | 105,000 |
Voltage (kV) | 300 | 300 |
Electron exposure (e–/Å2) | 52.0 | 52.0 |
Defocus range (μm) | −1.2 ~ -2.5 | −1.2 ~ -2.5 |
Pixel size (Å) | 0.85 | 0.85 |
Symmetry imposed | C1 | C1 |
Initial particle projections (no.) | 2,676,089 | 2,043,778 |
Final particle projections (no.) | 167,941 | 100,083 |
Refinement | ||
Initial model used (Alpha Fold database code) | AF-Q96P68-F1 | AF-Q96P68-F1 |
Map resolution (Å) | 3.16 | 2.84 |
FSC threshold | 0.143 | 0.143 |
Model composition | ||
Non-hydrogen atoms | 9097 | 9103 |
Protein residues | 1153 | 1152 |
R.m.s. deviations | ||
Bond lengths (Å) | 0.004 | 0.004 |
Bond angles (°) | 0.973 | 0.596 |
Validation | ||
MolProbity score | 1.69 | 1.60 |
Clashs core | 6.85 | 3.70 |
Ramachandran plot | ||
Favored (%) | 95.52 | 96.66 |
Allowed (%) | 4.48 | 3.34 |
Outliers(%) | 0.00 | 0.00 |
