Fig. 1: T cells are recruited to the airway after tobacco smoke exposure. | Nature Communications

Fig. 1: T cells are recruited to the airway after tobacco smoke exposure.

From: Comprehensive profiling of smoke-induced T cells in mice implicates clonal γδT17 cells as a hallmark of COPD

Fig. 1

Mice were sacrificed at 0 months (n = 5), 2 months (n = 4), and 6months (n = 4) after tobacco smoke exposure. T cells from the airway, the area between the airway and parenchyma (air-par), parenchyma, vasculature, and spleen were analyzed by fluorescence-activated cell sorting (FACS). a To localize T cells in the respiratory tract after tobacco smoke exposure, 0.25 mg of fluorochrome-conjugated CD45 and 0.5 mg of fluorochrome-conjugated CD90.2 antibodies were administered intranasally (i.n.) and intravenously (i.v.), respectively, as described in the Methods. The frequency and/or numbers of T cells (b) or T cell subsets (c) are shown. Data are mean \(\pm \,\)SEM. One-way ANOVA was used to compare differences among multiple groups when data followed a normal distribution, whereas the non-parametric Kruskal–Wallis test was applied for data that did not meet this assumption. NS not significant, Air Airways, Air-par the airway-parenchyma interface, Par parenchyma, Spl Spleen, CON air control group, 2 M, 2-month tobacco smoke exposure group; 6 M, 6-month tobacco smoke exposure group.

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