Fig. 4: Tolerance and susceptibility of cydA and icl1 CRISPRi knockdown strains to anti-tubercular drugs. | Nature Communications

Fig. 4: Tolerance and susceptibility of cydA and icl1 CRISPRi knockdown strains to anti-tubercular drugs.

From: Divergent transcriptional regulation of redox-homeostasis and permeability modulate rifampicin tolerance and sensitivity in Mycobacterium tuberculosis

Fig. 4

TTR based time-kill kinetic assays of (A–F) cydA knockdown strain performed against A bedaquiline (BDQ), B telacebec (Q203), C isoniazid (INH), D moxifloxacin (MXF) E ethambutol (EMB) and F rifampicin (RIF). G–L Time-kill kinetics of icl1 knockdown strain against G bedaquiline (BDQ), H telacebec (Q203), I isoniazid (INH), J moxifloxacin (MXF) K ethambutol (EMB) and L rifampicin (RIF). Data from biological triplicates are shown. High CFU shown in the INH treated cultures are drug resistant mutants appeared during the late time points (C,I). Kill curves of cydA and icl1 are represented with different color dots for clarity. Line indicates mean CFU obtained from three biological replicates. *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001, statistical significance estimated using a two-tailed unpaired t-test, GraphPad Prism 9.1.

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