Fig. 2: Conformational dynamics of MAD1 analogues.
From: Retro-inversion imparts antimycobacterial specificity to host defense peptides
CD spectra of MAD1 and MAD1-I (a), or MAD1-R and MAD1-RI (b), in phosphate buffer. c Secondary structure assignment for the MAD1 (top) and MAD1-RI (bottom) peptides. Structural classification was performed using the Beta Structure Selection (BeStSeL) algorithm. d–i Left: Full CD spectra and Right: Ellipticity changes for the β-sheet (θ212nm) and tryptophan-zipper (θ228nm) structural features, for MAD1 (d, f, h) and MAD1-RI (e, g, i) as a function of SDS concentration (d, e), NaCl concentration (f, g) and temperature (h, i). Representative spectra and analyses produced from n = 3 technical replicates. Source data are provided as a Source Data file.
