Fig. 4: Intracerebroventricular injection of AAV-Lec-SK3 alleviates cognitive deficits in 5×FAD mice. | Nature Communications

Fig. 4: Intracerebroventricular injection of AAV-Lec-SK3 alleviates cognitive deficits in 5×FAD mice.

From: Chimeras co-targeting antigens and FcγRIIb trigger degradation of extracellular soluble proteins and pathological aggregates

Fig. 4: Intracerebroventricular injection of AAV-Lec-SK3 alleviates cognitive deficits in 5×FAD mice.

a Schematic diagram of AAV virus sequence. b Binding to FcγRIIb (left) and antibody concentrations (right) in mouse brain homogenates were measured via ELISA at 20 days after i.c.v. injection of AAV (n = 4 mice per group). Data are presented as means ± SD. Statistical significance was determined by Unpaired t test. c Experimental timeline for 2-month-old male WT or 5×FAD mice receiving bilateral i.c.v. injections of saline, AAV-Lec-SK3, AAV-Lec, or AAV-GFP. Spontaneous alternation rate (d), exploration frequency (e), and dwelling duration (f) in the novel arm of the Y-maze were assessed at 20 and 60 days post-injection of saline, AAV-Lec-SK3, AAV-Lec, or AAV-GFP (n = 6 mice per group). g Schematic representation of NOR test. h Discrimination index (%) of the NOR test was determined following the injection of saline, AAV-Lec-SK3, AAV-Lec, or AAV-GFP (n = 6 mice per group). Data are presented as means ± SEM. Statistical significance was determined by one-way ANOVA with Dunnett’s multiple comparisons test (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, the exact P value were provided in Supplementary Table 7). Panel (a) was created in BioRender. Zhao, J. (2025) https://BioRender.com/zteancb. Panel (c) was created in BioRender. Zhao, J. (2025) https://BioRender.com/53ug670.

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