Fig. 1: The expression of TGFB1I1, encoding Hic-5, is induced by mechanical compression, rhTGF-β1, and bronchoconstriction in human airway basal cells.
From: Hic-5 drives epithelial mechanotransduction promoting a feed-forward cycle of bronchoconstriction
A Compression-induced TGFB1I1 mRNA expression in HBE cells from scRNA-seq data. Expression plots show normalized TGFB1I1 mRNA levels at 3 and 24 h following mechanical compression (30 cm H2O). TGFB1I1 mRNA expression was significantly increased by mechanical compression (mean ± SEM, n = 6 HBE cell donors). P values: one-way ANOVA with Holm–Šídák post hoc correction. B TGFB1I1 mRNA expression in HBE cells was measured at 24 h post-compression. TGFB1I1 mRNA expression was significantly increased by mechanical compression (mean ± SEM, n = 4 HBE cell donors) P value: two-way ANOVA. C TGFB1I1 mRNA expression in HBE cells was determined following exposure to rhTGF-β1 (10 ng/ml) for 24 h. Stimulation with rhTGF-β1 significantly increased TGFB1I1 expression (mean ± SEM, n = 6 HBE cell donors). P value: two-way ANOVA. In A–C, each symbol represents an individual donor: open circles for control and closed circles for compression or rhTGF-β1. D Reanalysis of published scRNA-seq data from human bronchial biopsies17. The plot presents the percentage of cells (y-axis) in each epithelial cell type (x-axis) expressing TGFB1I1 for individual patients (small dots) after challenge with a diluent control (gray circles) or an allergic antigen (red circles). P value: MAST likelihood-ratio test, two-sided56. (n = 4 patients). E Reanalysis of published scRNA-seq data from the Human Lung Cell Atlas18 comparing mean expression of TGFB1I1 transcript (y-axis) between airway basal and non-basal (other lung epithelial) cells (x-axis) for each individual donor (small dots). P value: Wilcoxon rank-sum test. (n = 37 donors) In D, E, large dots represent the mean of individual human donors, mean ± SEM. F Uniform manifold approximation and projection (UMAP) embedding of published scRNA-seq profiles of human airway epithelial cells after 14 days of air-liquid interface (ALI) differentiation, downloaded from GEO accession (GSM2772992)19, colored by expression of key marker genes.
