Fig. 4: Mechanical compression induces Hic-5, which then drives basal cell stress fiber formation and apical cell elongation.
From: Hic-5 drives epithelial mechanotransduction promoting a feed-forward cycle of bronchoconstriction
A, B Representative immunofluorescence (IF) images of Hic-5 (red), nuclei (blue), and F-actin (green) in Hic-5 WT and Hic-5 KD HBE cells at 24 h post-compression. Mechanical compression induced the expression of Hic-5 protein, which localized to the cytosol in Hic-5 WT HBE cells but was abolished in Hic-5 KD cells (A). Dotted boxes indicate regions shown expanded twofold in the insets presented in the upper right corner. Scale bars = 20 μm (10 μm in insets). Mechanical compression induced apical cell elongation and basal cell stress fiber formation in Hic-5 WT HBE cells but not in Hic-5 KD HBE cells (B). Scale bar = 20 μm, n = 5 HBE cell donors.
