Fig. 5: Endothelin-1 expression and secretion depend on Hic-5.
From: Hic-5 drives epithelial mechanotransduction promoting a feed-forward cycle of bronchoconstriction
A, B The validation of the top two ranked DE genes between Hic-5 WT and Hic-KD were quantified by qPCR. Compression-induced expression of PDGF (A) and EDN1 (B) was observed in Hic-5 WT HBE cells but was abolished in Hic-5 KD cells (mean ± SD, n = 3 HBE cell donors). P values: two-sided Student’s t-test. C, D Secretion of endothelin-1 (ET-1) by HBE cells into the basolateral conditioned media was determined by ELISA at 24 h post-compression (C) and post-TGF-β1 treatment (D). Hic-5 deficiency (Hic-5 KD) attenuated ET-1 secretion that was induced by both compression and TGF-β1 (mean ± SD, n = 5 HBE cell donors in (C) and n = 3 HBE cell donors in (D). P values: one-way ANOVA with Bonferroni post hoc correction. In A–D, each symbol represents an individual donor: open circles for control and closed circles for compression or rhTGF-β1 as indicated.
