Fig. 1: Schematic representation of the phage infection cycle in SCs. | Nature Communications

Fig. 1: Schematic representation of the phage infection cycle in SCs.

From: A synthetic cell phage cycle

Fig. 1: Schematic representation of the phage infection cycle in SCs.

A The goal is to recapitulate key infection steps: phage adsorption, DNA entry, genome expression and replication, virion assembly, and SC lysis, releasing progeny phages into the external solution. B Schematic representation of RdLPS SC composition. The SCs consist of liposomes made from phospholipids (POPC and PE-PEG5000) and RdLPS. The RdLPS are exposed on the outer leaflet to enable phage T7 binding and subsequent infection. A cell-free expression (CFE, yellow) system devoid of DNA, messenger RNA, and phage DNA and proteins is encapsulated into the liposome to allow for phage genome expression upon DNA entry, initiating replication and virion production. The SCs are made in a buffered solution (blue).

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