Fig. 3: Site-saturation functional screens of PALB2 variants in the WD40 domain. | Nature Communications

Fig. 3: Site-saturation functional screens of PALB2 variants in the WD40 domain.

From: Site-saturation functional screens identify PALB2 missense variants associated with increased breast cancer risk

Fig. 3

Amino acid function maps that comprise the entire WD40 domain of PALB2 spanning 331 amino acid residues from p.Q856 to p.S1186 (top of each map). Each map corresponds to a distinct WD40 variant library (left and Supplementary Fig. 1). The maps show depletion scores for 6419 PALB2 missense and nonsense variants as generated by Enrich2, n = 3 biological replicates. Amino acid characteristics for all variants are indicated (left of each map). Dark red squares represent variants that were depleted in PARPi-treated conditions versus untreated conditions, n = 3 technical replicates. Blue squares represent variants that were enriched. Grey squares represent variants that were either missing in the libraries or filtered out during the analysis. Grey dots represent the original (wild-type) amino acids. Data for all variants were normalized to WT PALB2, which was set to ‘0’, and to the average of the nonsense variants per library, which was set to ‘−1’. Source data are provided as a Source Data file.

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