Fig. 5: Single-cell sequencing analysis elucidates the developmental expression patterns of KCC2 across all major neuronal types, revealing that Sst-positive interneurons exhibit earlier KCC2 expression than other neuronal subtypes. | Nature Communications

Fig. 5: Single-cell sequencing analysis elucidates the developmental expression patterns of KCC2 across all major neuronal types, revealing that Sst-positive interneurons exhibit earlier KCC2 expression than other neuronal subtypes.

From: Functional KCC2 expression marks an evolutionarily conserved population of early-maturing interneurons in the perinatal cortex

Fig. 5

a Above: Sankey plot depicting scheme of neuronal families of Glutamatergic and GABAergic neurons comprised in the single cell analyses. 27 sub types of neurons and INs were present in the data set. Below: Cortico-stratal positions and schematic morphologies of discrete Glutamatergic and GABAergic neurons comprised in the single cell analyses. b Above: Graphical illustration of mice in embryonic, postnatal, adolescent, and late adult stages, depicting the whole course of development. Below: Heatmap quantifying absolute expression levels of Kcc2 gene Slc12a5 across 27 cortical neuronal populations, including glutamatergic and GABAergic neurons. The expression of Kcc2 gene Slc12a5 is tracked through 12 time points from embryogenesis to adulthood: E11.5, E12.5, E13.5, E14.5, E15.5, E16.5, E17.5, E18.5, P0, P2, P5, and adult (P60+). Prominent expression of Slc12a5, matching adult levels, is seen in four-layer 5/6 SST positive IN populations at birth on postnatal day 0 (T-Martinotti, Fanning Martinotti, Non-Martinotti, and Innervating cell) derived from single cell sequencing analysis utilizing a powerful bioinformatic atlas. Panel b inset created in BioRender. Srinivasan, R. (2025) https://BioRender.com/ac2n1el.

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