Fig. 4: The catalytic mechanism of bacteria-derived P450scc.
From: Unveiling cytochrome P450 enzymes that catalyze steroid side-chain cleavage in bacteria
a The structures of the hydroxylated substrates of CHO used in this study. b UPLC analysis of in vitro assays of CYP204A5 with monohydroxylated substrates of CHO in the presence of SeFdx1499/FdR0978 and NADPH at 254 nm. c The structures of the hydroxylated substrates of CHL used in this study. d UPLC-MS analysis of in vitro assays of CYP204A5 with hydroxylated substrates of CHL in the presence of SeFdx1499/FdR0978 and NADPH. The peaks with an asterisk represent unidentified dihydroxylation products of CHL that are not 20R,22R-DHC.
