Fig. 4: Structural determination and electrophysiological characterization of AtSLAH1.
From: Structural insights into the coordinated regulation of the SLAH family in Arabidopsis thaliana
a Cryo-EM map of the homotrimeric SLAH1-tRNA complex, with each protomer in a different color and tRNA in red; b Ribbon model of the AtSLAH1-tRNA complex, showing both transmembrane and intracellular views; c Ribbon model of a SLAH3 protomer viewed from the cytosol, with labeled transmembrane helices; d SLAH1 channel translocation pathway, calculated using HOLE software, with key residues indicated. e Whole-cell current traces, I/C-V curves, and bar graph summary of CHO cells expressing SLAH1 (n = 5) or SLAH1 + OST1 (n = 5). Pipette solution containing 10 mM RNase A induced whole-cell currents significantly higher than background levels (n = 5). Data were represented as means ± SEM, and p-values were calculated using two-way ANOVA with Sidak’s multiple comparisons test (e, middle) and one-way ANOVA with Dunnett’s multiple comparisons test (e, right). *p < 0.05, **p < 0.01, ***p < 0.001.
