Fig. 5: tRNA inhibition of AtSLAH1 channel activity.
From: Structural insights into the coordinated regulation of the SLAH family in Arabidopsis thaliana
a Model and representative density map of tRNA; b The intracellular region of SLAH1, exhibiting strong positive electric potential, binds to negatively charged tRNA. In contrast, the top view of SLAH1 displays a strong negative charge; c Hydrogen bond interactions between tRNA and the highly charged K and R residues in the intracellular area maintain the structural stability of tRNA; d Representative whole-cell current traces and I/C-V curves of CHO cells overexpressing SLAH1 + OST1 (n = 5) or SLAH1(K/R mutant)+OST1 (n = 11). Data were represented as means ± SEM. e Representative whole-cell current traces and I/C-V curves of Arabidopsis protoplast overexpressing SLAH1 + OST1 (n = 3) or SLAH1(K/R mutant)+OST1 (n = 5). Data were represented as means ± SEM. f Overexpression of Arabidopsis mutant AtSLAH1(K/R mutant) enhanced ABA-induced stomatal closure more than AtSLAH1 in Nb leaves. Data are means ± SD (n = 50), and p-values were calculated using two-way ANOVA with Sidak’s multiple comparisons test (d) and two-way ANOVA with Tukey’s multiple comparisons test (e). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars, 5 μm.
