Fig. 6: Structural comparison and ion permeation pathway of SLAH family proteins.
From: Structural insights into the coordinated regulation of the SLAH family in Arabidopsis thaliana
a Reversal potential (Erev) shifts of SLAH3 under different bath conditions: high NaCl (blue) (n = 4), low NaCl (red) (n = 5), and low Na+ (green) (n = 5). Data were represented as means ± SEM. b Representative whole-cell current traces and I–V curves recorded at 0 min and 10 min from blank CHO cells (n = 6) and CHO cells transfected with SLAH1 + OST1 (n = 12). Pipette solution contained 10 mM RNase A. c Bar graph showing the shift in SLAH1 reversal potential between 0 min and 10 min (ΔErev) induced by 10 mM RNase A included in the pipette solution (n = 12), and blank CHO cells (n = 6). Data were represented as means ± SEM, and p-values were calculated using two-tailed unpaired t-test. **p < 0.01 (SLAH1 compared with Blank cell). Data were represented as means ± SEM. d Whole-cell current traces, I/C-V curves, and bar graph summary of CHO cells expressing WT SLAH1 (n = 4), R47A (n = 4), or R198A mutants (n = 5). Data were represented as means ± SEM. P-values were calculated using two-way ANOVA with Dunnett’s multiple comparisons test (d, middle). ***p < 0.001 (R47A compared with SLAH1); ###p < 0.001 (R198A compared with SLAH1). P-values were calculated using one-way ANOVA with Dunnett’s multiple comparisons test (d, right). **p < 0.01 (R47A and R198A compared with SLAH1).
