Fig. 5: Nanchung pentamer assembly and mechanism for insect TRPV function.
From: Visualizing insecticide control of insect TRP channel function and assembly
a Representative 2D class averages Nan incubated with AP briefly before extraction, showing side views (upper) and bottom-up views (lower) of various oligomeric states. Angle of protomer contact is estimated by the red lines. b Comparison of tight junction contact angle and contact area (bolded surface), AP binding (as red sticks), and contact angle for Nan-Iav-CaM (upper panel) and Nan+AP pentamer (lower panel). The bracketed area (*) indicates interior contact comparison for Nan-Iav-CaM tight junction versus Nan+AP pentamer. c Cartoon representation of the AP binding sites of Nan-Iav tetramers and Nan pentamers with dashed lines indicating possible hydrogen bonds. d [3H]-AP binding to WT and mutant Nan-only membranes showing dissociation constants (Kd). Data are plotted as mean ± standard deviation (n = 3 technical replicates). e A model for AP-induced pentamerization of Nan in membranes with excess Nan to Iav. Source data are provided as a Source data file.
