Fig. 3: The primary P. copri metabolite, succinate, recapitulates the P. copri promotion of iWAT browning.
From: Prevotella copri promotes white adipose browning and ameliorates adiposity
a Targeted metabolomic analysis of organic acids in the culture supernatant of P. copri (n = 6 biological replicates). b, c The succinate content in fecal (b) (n = 6 biological replicates for HFD Control group and n = 7 biological replicates for HFD P. copri group) and cecal contents (c) (n = 6 biological replicates) of mice from HFD Control and HFD P. copri groups. d Experimental scheme for e-v. 8-week-old C57BL/6 J mice were given normal saline (HFD Control), 0.1 g/kg BW sodium succinate (SS) (HFD 0.1 g/kg BW SS), or 0.25 g/kg BW SS (HFD 0.25 g/kg BW SS) for 8 weeks under HFD feeding. e Body weight at 16 weeks old (n = 9 mice). f Representative pictures of body composition scanning of mice following 8 weeks’ treatment (n = 6 mice). g Fat index (%) (n = 6 mice). h Lean mass (n = 6 mice). i Representative images of iWAT. j iWAT weight (n = 9 mice). k Ratio of iWAT weight to body weight (n = 9 mice). l H&E-stained iWAT section (Scale bars, 50 μm), (n = 3 biological replicates). m Adipocyte sizes in iWAT (n = 3 biological replicates). n Rectal body temperature (n = 9). o-r CO2 production (o), O2 consumption (p) RER (q) and heat production (r) curves between HFD Control and HFD 0.25 g/kg BW SS groups (n = 4 mice), the data were analyzed by covariance (ANCOVA). s Relative mRNA levels of genes related to thermogenesis, lipogenesis and lipolysis in iWAT (n = 6 biological replicates). t, u Western blot analysis (t) and quantification (u) of the protein contents of thermogenic and lipogenic markers in iWAT (n = 4 biological replicates). v Representative immunofluorescence images of UCP1 (Red) from the iWAT section (Scale bars, 40 μm), (n = 3 independent experiments). Values are mean ± SEM. Statistical analysis was performed using two-tailed Mann-Whitney U test for b-c One-way ANOVA with LSD post hoc test for e, j-k, n, One-way ANOVA with Dunnett’s T3 post hoc test for m Kruskal-Wallis test followed by Dunn’s post hoc test for (g, h, s, u). Source data are provided as a Source Data file.
