Fig. 4: SUCNR1 is required for P. copri or succinate promotion of iWAT browning.
From: Prevotella copri promotes white adipose browning and ameliorates adiposity
a Relative Sucnr1 mRNA level between iWAT, eWAT and BAT in WT mice (n = 8 biological replicates). b Relative Sucnr1 mRNA level in iWAT of WT C57BL/6 J mice treated with normal saline, P. copri, or SS uder HFD conditions (n = 7 biological replicates). c-d Western blot analysis (c) and quantification (d) of the protein expression of SUCNR1 in iWAT of WT C57BL/6 J mice, with β-actin serving as a loading control (n = 4 biological replicates). e Experimental scheme in C57BL/6J-Sucnr1-/- mice for f-s. 8-week-old C57BL/6J-Sucnr1-/- mice were given normal saline (HFD Control), 108 CFU/kg BW P. copri (HFD P. copri), or 0.25 g/kg BW SS (HFD SS) for 8 weeks under HFD conditions. f Body weight of 16-week-old C57BL/6J-Sucnr1-/- mice following 8 weeks’ treatment (n = 8 mice). g Representative images of iWAT. h iWAT weight (n = 8 mice). i Ratio of iWAT weight to body weight (n = 8 mice). j H&E-stained iWAT sections (Scale bars, 50 μm), (n = 3 biological replicates). k Adipocyte sizes in iWAT (n = 3 biological replicates). l Rectal body temperature (n = 8 mice). m–p CO2 production (m) O2 consumption (n) RER (o) and heat production (p) curves (n = 4 mice for HFD Control group, n = 3 mice for HFD P. copri and HFD SS groups), the data were analyzed by covariance (ANCOVA). q Relative mRNA levels of Ucp1, Tbx1, Ap2, and Cpt1 in iWAT of Sucnr1-/- mice (n = 6 biological replicates). r, s Western blot analysis (r) and quantification (s) of the protein contents of thermogenic and lipogenic markers in iWAT (n = 3 biological replicates). Values are mean ± SEM. Statistical analysis was performed using One-way ANOVA with LSD post hoc test for k, Kruskal-Wallis test followed by Dunn’s post hoc test for a, b, d, f, h-i, l, q, s. Source data are provided as a Source Data file.
