Fig. 3: Auxin transport and signalling are impaired in the spl-1 mutant ovule.

pPIN1::PIN1:GFP expression in wild type (A, B) and spl-1 (C, D) ovules at stages 2-I (A, C) and 2-III (E, H).DR5v2 expression in wild type (E, F) and spl-1 (G, H) ovules at stages 2-I (E, G) and 2-III (F, H). R2D2 reporter line in wild type (I, J) and spl-1 (K, L) ovules at stage 2-III, showing mDII-tdTOMATO (I, K) and DII-VENUS (J, L) accumulation. I–L, M Line graph showing the mean ± SEM of PIN1-GFP signal intensity in the L1 layer of wild type and spl-1 ovules at stages 2-I and 2-III. Measurements have been performed on 5 wild type and 5 spl-1 different ovules at stage 2-I and on 12 wild type and 10 spl-1 different ovules at stage 2-III. Source data are provided as a Source Data file. Asterisks represent the statistical significance as determined by student’s t-test, two-sided distribution, homoscedastic, confronting the mutant with the wild-type condition at each time point. ^***p ≤ 0.001. Exact P values for each comparison can be found in the Source Data file. Single measures are represented with dots in correspondence to the line ends. N Line graph showing the mean ± SEM of the tdTOMATO signal intensity, from the DR5v2 reporter, in the L1 layer of wild-type and spl-1 ovules at stages 2-I and 2-III. Measurements have been performed on 6 wild-type and 4 spl-1 different ovules at stage 2-I and on 5 wild-type and 5 spl-1 different ovules at stage 2-III. Source data are provided as a Source Data file. Asterisks represent the statistical significance as determined by student’s t-test, two-sided distribution, homoscedastic, confronting the mutant with the wild-type condition at each time point. ^***p ≤ 0.001. Exact P values for each comparison can be found in the Source Data file. Single measures are represented with dots in correspondence to the line ends. nu nucellus, ch chalaza, fu funiculus, ii inner integument, oi outer integument, in integument, MMC megaspore mother cell. Scale Bars = 20 µm.