Fig. 5: shy2.6 expression in the nucellus impairs the MMC differentiation. | Nature Communications

Fig. 5: shy2.6 expression in the nucellus impairs the MMC differentiation.

From: SPOROCYTELESS/NOZZLE cooperates with MADS-domain transcription factors to regulate an auxin-dependent network controlling Megaspore-Mother-Cell differentiation

Fig. 5

Wild type ovules at stage 1-II (A), 2-I (B), 2-II (C) and 2-III (D). spl-1 ovules at stage 1-II (E), 2-I (F), 2-II (G) and 2-III (H). pSPL/NZZ:shy2.6 ovules at stage 1-II (I), 2-I (J), 2-II (K) and 2-III (L). shy2.6 expression in the wild-type nucellus impair the MMC differentiation, mimicking the spl-1 phenotype (G, H, K, L). M Bar plot showing the mean ± SEM of the percentage of ovules showing an MMC in wild type, spl-1 and pSPL/NZZ::shy2.6 lines. The analysis has been performed on 3, 4, 8, 9 and 5 different pistils, respectively, for the wild type (113 ovules observed in total), spl-1 (157 ovules observed in total), pSPL/NZZ::shy2.6 L1 (356 ovules observed in total), pSPL/NZZ::shy2.6 L2 (378 ovules observed in total) and spl-1 pSPL/NZZ::shy2.6 L3 (202 ovules observed in total). Source data are provided as a Source Data file. Letters above the bars indicate homogenous categories with p ≤ 0.05, as determined by one-way ANOVA with post-hoc Tukey HSD test. Exact P values for each comparison can be found in the Source Data file. Single measures are represented with dots in the bars. nu nucellus, ch chalaza, fu funiculus, ii inner integument, oi outer integument, in integument, MMC megaspore mother cell. Scale bars = 20 µm.

Back to article page