Fig. 6: Increasing ARF levels in the spl-1 nucellus partially restores the MMC differentiation. | Nature Communications

Fig. 6: Increasing ARF levels in the spl-1 nucellus partially restores the MMC differentiation.

From: SPOROCYTELESS/NOZZLE cooperates with MADS-domain transcription factors to regulate an auxin-dependent network controlling Megaspore-Mother-Cell differentiation

Fig. 6

Wild type ovules at stage 2-I (A) and 2-III (B). spl-1 ovules at stage 2-I (C) and 2-III (D). spl-1 pSPL/NZZ::MP ovules at stage 2-I (E) and 2-III (F). spl-1 pSPL/NZZ::ARF9 ovules at stage 2-I (G) and 2-III (H). While increasing MP expression only partially restores the MMC differentiation, pSPL/NZZ::ARF9 rescues the MMC differentiation in spl-1. I Bar plot showing the mean ± SEM of the percentage of ovules showing an MMC in wild type, spl-1 and spl-1 pSPL/NZZ::MP lines. The analysis has been performed on 5, 8, 12 and 9 different pistils for the wild type (150 ovules observed in total), spl-1 (304 ovules observed in total), spl-1 pSPL/NZZ::MP L1 (362 ovules observed in total) and spl-1 pSPL/NZZ::MP L2 (270 ovules observed in total). Source data are provided as a Source Data file. Letters above the bars indicate homogenous categories with p < 0.05, as determined by one-way ANOVA with post-hoc Tukey HSD test. Exact P values for each comparison can be found in the Source Data file. Single measures are represented with dots in the bars. J Bar plot showing the mean ± SEM of the percentage of ovules showing an MMC in wild type, spl-1 and spl-1 pSPL/NZZ::ARF9 lines. The analysis has been performed on 4, 4, 5, 4, 4 different pistils for the wild type (157 ovules observed in total), spl-1 (131 ovules observed in total), spl-1 pSPL/NZZ::ARF9 L1 (193 ovules observed in total), spl-1 pSPL/NZZ::ARF9 L2 (115 ovules observed in total) and spl-1 pSPL/NZZ::ARF9 L3 (177 ovules observed in total). Source data are provided as a Source Data file. Letters above the bars indicate homogenous categories with p ≤ 0.05, as determined by one-way ANOVA with post-hoc Tukey HSD test. Exact P values for each comparison can be found in the Source Data file. Single measures are represented with dots in the bars. nu nucellus, ch chalaza, fu funiculus, ii inner integument, oi outer integument, in integument, MMC megaspore mother cell. Scale bars = 20 µm.

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