Fig. 3: LC427 regulates LRBA-dependent signaling in T cells.
From: Targeting LRBA triggers CTLA4 degradation and antitumor immunity for cancer immunotherapy
A Volcano plots of LC427-regulated proteins in mouse T cells by the whole proteomics (n  =  3 biological replicates). B Heat map of proteins for differential signaling pathways (Orange upregulated, blue downregulated). C The upregulated GSEA signatures in mouse T cells with LC427 treatment. The expression level of these proteins in mouse T cells with LC427 treatment was measured by the whole proteomics (n  =  3 biological replicates). D Western blotting analyses determined protein expression of Lrba-dependent signaling in Lrba-Knockdown (shLrba) or Control-Knockdown (shCtrl) T cells with DMSO or LC427 treatment. The relative density values for each band beneath the corresponding Western Blot lanes and from two independent experiments (mean). Molecular weight markers (kDa) on the right of the relevant Western Blot images. E Western blotting analyses determined protein expression of LRBA-dependent signaling in CTLA4-/--T cells with DMSO or LC427 treatment. The relative density values for each band beneath the corresponding Western Blot lanes and from two independent experiments (mean). Molecular weight markers (kDa) on the right of the relevant Western Blot images. Statistical significance in (A) was assessed using two-way T-test analyses. Source data are provided as a Source Data file.
