Fig. 5: Slower-growing species A01 secretes public goods and promotes the growth of low-tolerance B01 via cross-feeding. | Nature Communications

Fig. 5: Slower-growing species A01 secretes public goods and promotes the growth of low-tolerance B01 via cross-feeding.

From: Slower-growing species promote interspecific cooperation and coexistence under acid stress through cross-feeding

Fig. 5

A Schematic diagram to verify that B01 can successfully grow again in public goods and A01 excretes public goods. Detailed steps are described in the method section. B OD values of B01 monocultures in the acidified CDMG liquid medium with (w/Pg.Supp., filled symbols) or without public goods (wo/Pg.Supp., open symbols). C Changes in the content of public goods over time were detected by HPLC when B01 was monocultured in 100 mM lactate-acidified CDMG liquid medium supplemented with public goods, including 6.0 mM pyruvate (right vertical axis), 2.7 mM glutamate, and 2.0 mM aspartate. D OD values of B01 monocultures in 100 mM lactate-acidified CDMG liquid medium supplemented with different concentrations of public goods (PG). The black-filled square in D was a reuse of the gray open square in B. Specific concentrations supplemented are shown in Table S4. E Biomass of mono- and co-cultured A01 and B01 in CDMG agar plates supplemented with the inhibitor 3-bromopyruvate. F HPLC-detected pyruvate, glutamate, and aspartate concentrations in extracts from co-culture regions of A01 and B01 in CDMG agar plates supplemented with 3-bromopyruvate. G Changes in biomass and glucose concentration (right vertical axis) over time when A01 was mono-cultured in 100 mM lactate-acidified CDMG liquid medium containing 25.0 mM glucose without adding public goods. H Content of public goods excreted by A01 into the medium was detected by HPLC over time when A01 was mono-cultured in 100 mM lactate-acidified CDMG medium without added public goods. The data in (B–H) represent the average of three biological replicates; see source data for SD. I Untargeted metabolomics to detect the relative intensity of cocultured metabolites in CDMG medium, as detailed in “Methods”. The color lines indicate the change curves for the pivotal metabolites (those with relative intensities <0.5), whereas the gray lines indicate the change curves for the other metabolites. Specific metabolites are listed in Table S5.

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