Fig. 6: Lactylation at Lys688 regulated by PCAF orchestrates the remodeling of BCL6 ubiquitination.
a Healthy CD4+ T cells were co-cultured with MCs for 4 days and BCL6 lactylation was detected. b Healthy CD4⁺ T cells were co-cultured with GSK2837808A pretreated-MCs for 4 days. c Healthy CD4⁺ T cells were co-cultured with LN-associated MCs transfected with shLDHA or shNC for 4 days. d Healthy CD4⁺ T cells were co-cultured with UK5099 (20 μM) pretreated-MCs for 4 days. e MCs were transfected with siMPC1, siNC or MPC1-overexpression plasmids (after SLE plasma stimulation). Healthy CD4⁺ T cells were co-cultured with MCs for 4 days. f HEK293T cells were transfected with HA-BCL6, HA-BCL6K379R, HA-BCL6K688R and HA-BCL6K379K688R plasmids. After stimulation with LacNa, HA-BCL6 was immunoprecipitated for detecting lactylation. g HEK293T cells were transfected with HA-BCL6, HA-BCL6K688R, HA-BCL6K534K628R, HA-BCL6K534K628K688R and Myc-Ub plasmids. After stimulation with LacNa, HA-BCL6 was immunoprecipitated to detect ubiquitination and lactylation. h HEK293T cells were transfected with HA-BCL6, HA-BCL6K688R, and Myc-Ub plasmids. After stimulation with LacNa, HA-BCL6 was immunoprecipitated to detect ubiquitination. i HEK293T cells were transfected with HA-BCL6, Flag-GCN5, Flag-PCAF, Flag-CBP, Flag-P300 and Myc-TIP60 plasmids. HA-BCL6 was immunoprecipitated for detecting lactylation. j Anti-CD3/28-activitated healthy CD4+ T cells were stimulated with LacNa, PCAF expression and the interaction of PCAF with BCL6 was detected. k HEK293T cells were transfected with HA-BCL6, HA-BCL6K688R, Flag-PCAF, shPCAF and Myc-Ub plasmids. After stimulation with LacNa, HA-BCL6 was immunoprecipitated to detect ubiquitination and lactylation. l Anti-CD3/28-activated healthy CD4⁺ T cells were transduced with shPCAF or shNC, stimulated with LacNa. m Healthy CD4⁺ T cells transduced with shPCAF or shNC were co-cultured with LN-associated MCs (n = 6 independent biological samples). n Mice were immunized with immunogenic self-DNA (n = 7 independent biological samples). The data are represented as the mean ± SEM. Normality and lognormality are assessed using the Shapiro-Wilk test. Two-tailed unpaired t test (n), two-tailed paired t test (m). Source data are provided as a Source Data file.
