Fig. 2: Spatial identification and visualization of stroma in human endometrium using Stereo-seq and scRNA-seq. | Nature Communications

Fig. 2: Spatial identification and visualization of stroma in human endometrium using Stereo-seq and scRNA-seq.

From: Decrypting spatiotemporal code of human endometrial receptivity

Fig. 2: Spatial identification and visualization of stroma in human endometrium using Stereo-seq and scRNA-seq.The alternative text for this image may have been generated using AI.

A UMAP visualization showing the re-clustering of stroma. proStr proliferating stromal cells, undiffStr undifferentiated stromal cells, diffStr differentiated stromal cells, deStr degrading stromal cells. B Heatmap showing the module correlations of stromal cell subclusters based on pairwise gene expression. C Expression of classical marker genes of stromal cell subclusters. D Cell cycle analysis of proStr, undiffStr, diffStr, and deStr in the endometrium. E Co-immunostaining of WT1 (green) and MKI67 (red) in human endometrium across the LH stages (n = 3). Nuclei are counterstained with DAPI (blue). F Stereo-seq visualization of different stroma subclusters within human endometrium. G Spatial expression of SFRP4, NR2F1, SERPING1, and THBS2 in human endometrium from LH2 to LH12 using Stereo-seq (bin20). H ISS of SFRP4, NR2F1, SERPING1, and THBS2 in human endometrium from LH2 to LH12 (n = 1).

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