Fig. 3: The oxidaiton products of taxadiene catalyzed by TteUPO mutants and the structural distribution of the investigated mutations in TteUPO.

A GC analysis of taxadiene oxidation catalyzed by mutants of TteUPO. * Under GC conditions, compounds 11 and 12 partially degrade, yielding products with retention times identical to taxadiene (1); B Structures and MS (EI) spectra of the identified products (9, 11-13, 15, and 16). Compound 8 is the product generated by the thermal transformation of 15 during GC detection (Supplementary Fig. 15), upon scale-up, compounds 10 and 14 exhibited significantly diminished yields (Supplementary Fig. 9D); C Cross-sectional view of the TteUPO (WT) structure, highlighting Leu208 (red); D Cross-sectional view of the L208A mutant structure, highlighting Ala208 (red); E Cross-sectional view of the TteUPO (WT) structure, highlighting Leu60 (red); F Cross-sectional view of the L60A mutant structure, highlighting Ala60 (red); G Structure of the L60A/F156L mutant, highlighting Leu156 (red); H Structure of the L60A/G159L mutant, highlighting Leu159 (red). Color representation of taxadiene: blue for ring A, green for ring B and yellow for ring C.