Fig. 4: Design TteUPO mutant’s toolbox for functional oxidation of taxadiene and taxanes with diverse oxidation states.

A Regioselectivity of mutants in the toolbox toward different taxanes. Definition of Regioselectivity: The peak area ratio between oxidation products at different positions on the taxane skeleton. Compound 15 or 16 is equally allocated between the C-6 position and combined C-11/C-12 positions. Unidentified products are excluded from the analysis; B Oxidation of Taxanes using TteUPO mutants in the constructed toolbox. The proportions of the depicted products relative to the total products were determined in a single-step enzymatic reaction. Y-axis Peak area ratio of illustrated compounds in the reaction mixture, with inclusion of unidentified products. Source data are provided as a Source Data file.