Fig. 5: De novo biosynthesis of oxidized taxadiene products using an engineered E. coli chassis.
From: Designing an oxidase toolbox for site-directed oxidation of taxanes
A Schematic of the biosynthesis pathway of taxadiene oxidation products in engineered E. coli. GGPPS: geranylgeranyl pyrophosphate synthase; TDS: taxadiene synthase. H2O2 was exogenously supplemented; B GC analysis of products from the engineered E. coli chassis. Heterologous expression of L208A and exogenous supplementation of H2O2 enabled the detection of products 3, 4, 5, and 21. C Yields of taxane oxidation products in the engineered E. coli chassis. The bars are mean of n =  3 independent experiments and error bars indicate s.d. Source data are provided as a Source Data file.
