Table 1 Comparison of the activity and selectivity of TteUPO and its mutants for taxadiene oxidation
From: Designing an oxidase toolbox for site-directed oxidation of taxanes
catalyst | Enzyme load (μg/mL) | TONa | Conv. (%)b | Product proportion (%) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
3 | 4 | 5 | 6 | 9 | 11 | 12 | 13 + 14 | 15 | 16 | Opc | ||||||
WT | 40 | 82 | 18.0 ± 0.7 | <1 | 11 | 85 | 4 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
L208A | 4 | 4355 | 95.6 ± 0.5 | 1 | 12 | 85 | 2 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | ||
L60A | 40 | 432 | 94.9 ± 0.4 | n.d. | <1 | 4 | n.d. | 15 | 16 | 9 | 4 | 31 | 2 | 18 | ||
L60A/F156A | 40 | 430 | 94.5 ± 0.7 | n.d. | n.d. | n.d. | n.d. | 2 | n.d. | n.d. | 10 | 6 | 51 | 31 | ||
L60A/F156L | 40 | 224 | 49.2 ± 0.3 | n.d. | n.d. | n.d. | n.d. | 42 | 27 | 22 | n.d. | 8 | n.d. | 1 | ||
L60A/G159L | 40 | 365 | 80.2 ± 0.2 | n.d. | n.d. | 2 | n.d. | n.d. | 66 | 19 | 2 | n.d. | n.d. | 11 | ||
L60A/G159L/L208A | 20 | 757 | 83.1 ± 2.5 | n.d. | n.d. | n.d. | n.d. | n.d. | 41 | 33 | 2 | n.d. | n.d. | 24 | ||
