Fig. 5: CPI-455 treatment improves mouse pregnancy outcome.

a A schematic representation exhibiting the addition of CPI-455 during mouse embryo development. Illustration was created in BioRender. Zhang, C. (https://biorender.com/b1074ci). b Representative images of blastocysts derived from IVF following the supplementation with different concentrations of CPI-455. Scale bars, 400 μm, 200 μm. 4x and 10x represent the microscopy magnification times. c The blastocyst formation rate under several conditions with different concentrations of CPI-455. Source data are provided as a Source Data file. Error bar: mean ± std. Two-tailed unpaired t tests were performed. d Upper: a schematic diagram showing how SRGs were identified upon CPI-455 treatment; lower: expression heatmap of the SRGs. N = 3 biological replicates for each condition. Illustration was created in PowerPoint. e Relative mRNA expression levels of several SRGs involved in oxidative stress, functions of ATP and mitochondrion. Source data are provided as a Source Data file. Error bar: mean ± std. Two-tailed unpaired t tests were performed. f The distribution of H3K4me3 signals around TSS of several SRGs validated by RT-qPCR (i.e., genes exhibited in Fig. 5e and Supplementary Fig. 5e). g, h Staining and quantification for ROS (g) and GSH (h) in IVF-conceived blastocysts with/without CPI-455 treatment. Scale bars, 60 μm. Source data are provided as a Source Data file. Error bar: mean ± std. Two-tailed unpaired t tests were performed. i A schematic diagram summarizing the crosstalk between H3K4me3 and oxidative stress as well as the potential mechanisms behind it. Illustration was created in BioRender. Zhang, C. (https://biorender.com/8za44jv). Source data are provided as a Source Data file.