Fig. 7: Defective adult neurogenesis in Celsr1-deficient mice.

a Freshly dissected mature brain depicting the sectioning plan and position (white line) of the section shown in (b). b Photograph of an autoradiography film from a parasagittal section hybridized with a Celsr1 riboprobe. Celsr1 mRNA is found in the subventricular zone (SVZ) and rostral migratory stream (RMS). c Photographs of dissected adult brains from control (left) and Celsr1 KO (right) mice. The olfactory bulbs (OB), and to a lesser extent the cerebellum, are reduced in the KO mice. d Nissl-stained sagittal section highlighting the subventricular zone and rostral migratory stream. The box area represents the brain region analyzed in (e). e Sagittal sections immunostained with anti-doublecortin (Dcx, green) antibodies. Neuroblasts populate the olfactory bulb and outline the rostral migratory stream in control, but not in Celsr1 KO mice. Observations without quantification have been validated and successfully reproduced in a minimum of five different animals. f Coronal hemisection of the mouse forebrain, indicating the region analyzed in (g) and (h) (boxed area). g BrdU staining at the rostral aspect of the lateral ventricle. h Quantification of the BrdU staining. Celsr1 KO mice have a higher density of BrdU-positive cells in the rostral SVZ (Ctrl: 120.3 ± 10.2 cells/mm²; KO: 191.4 ± 11.4 cells/mm²; p = 0.0001). i Coronal hemisection labeled with Cresyl Violet (Nissl), indicating the regions magnified in (j). j BrdU staining at the caudal aspect of the lateral ventricle. The white boxes indicate the region where the counting was performed. k quantification of the number of BrdU-positive cells at the caudal aspect of the lateral ventricle (Ctrl: 47.18 ± 6.8 cells/mm²; KO: 91 ± 8.8 cells/mm2; p = 0.0007). Note the enlarged ventricles in the Celsr1 KO mice. (n = 12 sections from three different animals for Ctrl and KO mice). Data were represented as mean ± SEM. Values were obtained by unpaired two-tailed Student’s t-test; ns not significant, *p < 0.05, **p < 0.01, and ***p < 0.001. CC corpus callosum, CPu caudate putamen (striatum), V ventricle. Scale bars, (b, c) 1 mm, (e) 800 μm, (g) 200 μm, (j) 400 μm. CC corpus callosum, CPu caudate putamen (striatum), fi fimbria of the hippocampus, V ventricle. Uncropped and unprocessed data are available as Supplementary Fig. 5. Source data are provided in the Source Data File.