Fig. 2: ZmCRYs mediate the blue light-regulated genes’ transcription in maize. | Nature Communications

Fig. 2: ZmCRYs mediate the blue light-regulated genes’ transcription in maize.

From: Blue-light-enhanced interaction between ZmCRY1s and GL2 modulates epidermal wax composition in Zea mays

Fig. 2: ZmCRYs mediate the blue light-regulated genes’ transcription in maize.

a Venn diagram showing the overlap between sets of DEGs from blue light treatment or not in B73 (B73_Blue vs. B73_Dark), and in cryq (cryq_Blue vs. cryq_Dark) at 28 °C. b Heatmap generated from ZmCRYs-mediated DEGs. c KEGG pathway enrichment analysis of blue light-upregulated genes. Significantly enriched KEGG pathways were defined as overrepresented terms in the blue light-upregulated gene set, identified using a one-sided (right-tailed) Fisher’s exact test with a liberal statistical significance threshold of P < 0.05. d RT-qPCR validates the already known blue light signaling pathway regulators and the up-regulated fatty acid biosynthesis enzymes detected in the transcriptome. Seven-day LD-grown B73 and Zmcry-q, pretreated with 48-h dark, then moved to blue light (20 μmol m−2 s−1) for a 2-h time course. ZmUBQ served as internal control. Error bars, SD of three biological replicates. Data are presented as mean values ± SD.

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